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smrtbell express template prep kit 2 0  (Pacific Biosciences)

 
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    Pacific Biosciences smrtbell express template prep kit 2 0
    Smrtbell Express Template Prep Kit 2 0, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/template/0+3+kit+prep+smrtbell/pm42321367-55-18-24
    Average 86 stars, based on 1 article reviews
    smrtbell express template prep kit 2 0 - by Bioz Stars, 2026-09
    86/100 stars

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    Construct:

    Article Title: A newly developed eGFP-traceable recombinant rhesus lymphocryptovirus, a tool to study viral infection and replication in vitro and ex vivo , confirms gp350 as key for viral entry.
    Article Snippet: Next, the fragmentation of 2 μg of isolated genomic DNA was conducted using the Covaris S220 ultrasonicator system (Matthew, NC), and the resulting sheared DNA sizes were analyzed using the Agilent 2011 bioanalyzer (Santa Clara, CA). .. SMRTbell libraries were constructed following the PacBio standard 20-kb template preparation protocol using the SMRTbell Template Prep Kit 1.0 from Pacific Biosciences (Menlo Park, CA). .. Briefly, the DNA was incubated with exonuclease VII (NEB) at 37°C for 15 min to remove single-stranded DNA, and any potentially damaged DNA was repaired using a DNA damage repair mix at 37°C for 20 min. Blunt-ended DNAs were treated with end-repair mix at 25°C for 5 min and ligated with 1 μM of annealed blunt adapters using 0.75 U/μL ligase at 25°C overnight; then, the ligase was inactivated by incubation at 65°C for 10 min. To remove failed ligation products, samples were treated with exonuclease III (NEB) and VII at 37°C for 1 h. To purify the DNAs and ligated products, 0.45× of AMPure PB Beads from Pacific Biosciences were applied.

    Article Title: Quantitative evaluation of microbiome sequencing resolution under varying experimental conditions using defined mock communities.
    Article Snippet: Amplicons were evaluated for ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS quality using a TapeStationTM system (Agilent Technologies), and equimolar pooling of all samples was performed before library construction. .. Libraries were constructed using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA, USA) according to the manufacturer's instructions for full-length 16S library preparation. .. Library size and concentration were assessed using the Qubit Fluorometer with the QubitTM 1X dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA, USA) and the TapeStation system.



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    Image Search Results


    Workflow for barcode amplicon library preparation This figure summarizes the workflow for barcode amplicon library preparation in this part. The left panel shows barcode enrichment from scRNA-seq cDNA by two rounds of PCR, followed by bead purification and quality control to generate the final Illumina barcode library. The middle panel shows spike-in RNA preparation, including spike-in template assembly, purification, in vitro transcription, DNA digestion, RNA purification, and quality control. The right panel shows input region library preparation, including reverse transcription, pooling and purification, pre-amplification PCR, and quality control to generate the final Illumina input-region library. Step numbers in red correspond to the detailed protocol steps.

    Journal: STAR Protocols

    Article Title: Protocol to produce and apply barcoded rabies virus for single-neuron input mapping in mice

    doi: 10.1016/j.xpro.2026.104537

    Figure Lengend Snippet: Workflow for barcode amplicon library preparation This figure summarizes the workflow for barcode amplicon library preparation in this part. The left panel shows barcode enrichment from scRNA-seq cDNA by two rounds of PCR, followed by bead purification and quality control to generate the final Illumina barcode library. The middle panel shows spike-in RNA preparation, including spike-in template assembly, purification, in vitro transcription, DNA digestion, RNA purification, and quality control. The right panel shows input region library preparation, including reverse transcription, pooling and purification, pre-amplification PCR, and quality control to generate the final Illumina input-region library. Step numbers in red correspond to the detailed protocol steps.

    Article Snippet: Spike-In template F , TAATACGACTCACTATAGGGAGTGAC AATGAAACCTACGTAGTGCAAAGAGA AGTGGCAGTTGCCAAATACAGCAACC TTGGTGGTGGCATGGACGAGCTGTAC AAGTAAGGTACCGGCCAT , Sangon Biotech.

    Techniques: Amplification, Purification, Control, In Vitro, Reverse Transcription

    Workflow for barcode amplicon library preparation This figure summarizes the workflow for barcode amplicon library preparation in this part. The left panel shows barcode enrichment from scRNA-seq cDNA by two rounds of PCR, followed by bead purification and quality control to generate the final Illumina barcode library. The middle panel shows spike-in RNA preparation, including spike-in template assembly, purification, in vitro transcription, DNA digestion, RNA purification, and quality control. The right panel shows input region library preparation, including reverse transcription, pooling and purification, pre-amplification PCR, and quality control to generate the final Illumina input-region library. Step numbers in red correspond to the detailed protocol steps.

    Journal: STAR Protocols

    Article Title: Protocol to produce and apply barcoded rabies virus for single-neuron input mapping in mice

    doi: 10.1016/j.xpro.2026.104537

    Figure Lengend Snippet: Workflow for barcode amplicon library preparation This figure summarizes the workflow for barcode amplicon library preparation in this part. The left panel shows barcode enrichment from scRNA-seq cDNA by two rounds of PCR, followed by bead purification and quality control to generate the final Illumina barcode library. The middle panel shows spike-in RNA preparation, including spike-in template assembly, purification, in vitro transcription, DNA digestion, RNA purification, and quality control. The right panel shows input region library preparation, including reverse transcription, pooling and purification, pre-amplification PCR, and quality control to generate the final Illumina input-region library. Step numbers in red correspond to the detailed protocol steps.

    Article Snippet: Spike-In template R , TTTTTTTCTCGACTGAAATGCTTAGAT GACCCAGCCCTCAATAGGGCCGCCT CGGCCNNNTGNNNACNNNATNNNG CNNNTGNNNACNNNGGCCGTAATG GCCGGTACCTTA , Sangon Biotech.

    Techniques: Amplification, Purification, Control, In Vitro, Reverse Transcription